RESUMO
Phytoplasmas manipulate host plant development to benefit their invasion and insect vector colonization. However, the virulence factors and mechanisms underlying small leaf formation caused by jujube witches' broom (JWB) phytoplasmas remain largely unknown. Here, effectors SJP1 and SJP2 from JWB phytoplasmas were identified to induce small leaf formation in jujube. In vivo interaction and expression assays showed that SJP1 and SJP2 interacted with and stabilized ZjTCP2. Over-expression of SJP1 and SJP2 in jujube induced ZjTCP2 accumulation. In addition, the abundance of miRNA319f-1 was significantly repressed in leaves of SJP1 and SJP2 transgenic jujube plants and showed the opposite pattern with its target ZjTCP2, which was consistent with that in the diseased leaves. Overexpression of ZjTCP2 in Arabidopsis promoted ectopic leaves arising from the adaxial side of cotyledons and reduced the leaf size. Constitutive expression of the miRNA319f-1 precursor in the 35S::ZjTCP2 background reduced the abundance of ZjTCP2 mRNA and reversed the cotyledon and leaf defects in Arabidopsis. Therefore, these observations suggest that effectors SJP1 and SJP2 induced small leaf formation, at least partly, by interacting with and activating ZjTCP2 expression both at the transcriptional and protein levels, providing new knowledge about small leaf formation caused by phytoplasmas in woody plants.
RESUMO
Tumor metabolic reprogramming and epigenetic modification work together to promote tumorigenesis and development. Protein lysine acetylation, which affects a variety of biological functions of proteins, plays an important role under physiological and pathological conditions. Here, through immunoprecipitation and mass spectrum data, we show that phosphoglycerate mutase 5 (PGAM5) deacetylation enhances malic enzyme 1 (ME1) metabolic enzyme activity to promote lipid synthesis and proliferation of liver cancer cells. Mechanistically, we demonstrate that the deacetylase SIRT2 mediates PGAM5 deacetylation to activate ME1 activity, leading to ME1 dephosphorylation, subsequent lipid accumulation and the proliferation of liver cancer cells. Taken together, our study establishes an important role for the SIRT2-PGAM5-ME1 axis in the proliferation of liver cancer cells, suggesting a potential innovative cancer therapy.
Assuntos
Neoplasias Hepáticas , Sirtuína 2 , Humanos , Sirtuína 2/genética , Sirtuína 2/metabolismo , Metabolismo dos Lipídeos , Fosfoglicerato Mutase/genética , Fosfoglicerato Mutase/metabolismo , Proliferação de Células , Lipídeos , Acetilação , Fosfoproteínas Fosfatases/metabolismo , Proteínas Mitocondriais/metabolismoRESUMO
Klebsiella pneumoniae is one of the main pathogenic bacteria that causes nosocomial infections, such as pneumonia, urinary tract infection, and sepsis. Therefore, the rapid and accurate detection of K. pneumoniae is important for the timely treatment of infectious patients. This study aimed to establish a loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of K. pneumoniae-specific gene ureR_1 (Gene ID: 11847803). The ureR_1 gene was obtained through local and online BLAST, and the specific primers were designed for its detection. Positive reactions were observed on all 140 K. pneumoniae clinical isolates while all the 82 non-K. pneumoniae clinical isolates were negative. Plasmids with the specific gene and the mouse blood with K. pneumoniae were used for sensitivity analysis. The detection limit of the LAMP was 1 bacterium/reaction. The results showed that the LAMP targeted to ureR_1 is a fast, specific, sensitive, inexpensive, and suitable method for the detection of K. pneumoniae.
Assuntos
Genes Bacterianos , Klebsiella pneumoniae/genética , Técnicas de Amplificação de Ácido Nucleico/métodos , Primers do DNA/genética , Primers do DNA/isolamento & purificação , Klebsiella pneumoniae/isolamento & purificação , Limite de Detecção , Plasmídeos/genética , Plasmídeos/isolamento & purificação , Reação em Cadeia da Polimerase/métodos , Reprodutibilidade dos Testes , Análise de Sequência de DNA , Temperatura , Fatores de TempoRESUMO
Klebsiella pneumoniae is one of the main pathogenic bacteria that causes nosocomial infections, such as pneumonia, urinary tract infection, and sepsis. Therefore, the rapid and accurate detection of K. pneumoniae is important for the timely treatment of infectious patients. This study aimed to establish a loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of K. pneumoniae-specific gene ureR_1 (Gene ID: 11847803). The ureR_1 gene was obtained through local and online BLAST, and the specific primers were designed for its detection. Positive reactions were observed on all 140 K. pneumoniae clinical isolates while all the 82 non-K. pneumoniae clinical isolates were negative. Plasmids with the specific gene and the mouse blood with K. pneumoniae were used for sensitivity analysis. The detection limit of the LAMP was 1 bacterium/reaction. The results showed that the LAMP targeted to ureR_1 is a fast, specific, sensitive, inexpensive, and suitable method for the detection of K. pneumoniae.
